AmpliGene’s Smart POCT system – AmpEZ® is a versatile technology that lends itself for broad range of markets from large clinical laboratory settings, to Point-of-Care (POC).

The technology is ideal for DNA/RNA testing in human in vitro diagnostics (IVD), plant, GMO, Veterinary, Aquaculture, poultry or Biodefence. Studies have indicated that AmpEZ® outperforms other isothermal technologies and overcomes shortcomings of PCR.

AmpEZ® technology answers to increasing demands on faster and more cost-effective testing methods.

The novel AmpEZ® technology

  • Loop Mediated Isothermal Amplification (AmpEZ®)
  • Amplification of nucleic acids at a constant Temperature.
  • Both DNA and RNA targets can be detected .
  • Minimal sample preparation is required, no need to extract DNA
  • Ability to multiplex the reaction and detect amplicons individually
  • Features to minimize non-specific amplification
  • Real-time fluorescent detection, no post amplification steps, eliminating contamination
  • Easy instrumentation: in the field & POC as well as centralized settings

AmpEZ®- Benefits

Sensitivity Very low formation of artefacts allows for confidence In positive reactions with low target copies or long Run times
Specificity
  • Anneal curve analysis possible
  • Even 1-2 bp resolution
Speed
  • Rapid reaction
  • Hands-on-time short due to low sample prep requirements
Robustness
  • Tolerate high protein and high salt
  • Temperature exact level not critical
  • Lyophilsed Reagents available.
Multiplexability
  • Multiple different probe/detection chemistry variants developed, some proprietary